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Journal: Cell reports

Article Title: TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly

doi: 10.1016/j.celrep.2023.112577

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Single-particle tracking was carried out in MATLAB 2019a using a batch parallel-processing version of SLIMfast modified to allow the input of TIFF files (kindly provided by Xavier Darzacq and Anders Hansen), an implementation of the Multiple-Target-Tracing algorithm, with the following settings: Exposure Time = 10 ms, NA = 1.49, Pixel Size = 0.16 μm, Emission Wavelength = 664 nm, D max = 5 μm 2 /s, Number of gaps allowed = 2, Localization Error = 10 −5 , Deflation Loops = 0.

Techniques: Recombinant, Western Blot, Knock-Out, Software, SYBR Green Assay, Staining